SI-249

General

Cell Line

hPSCreg Name
RGIe148-A
Alternative name(s)
SI-249
Cell line type Human embryonic stem cell (hESC)
Last update 31st August 2022
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Provider

Generator Reproductive Genetics Institute (RGI)
Derivation country United States

External Databases

Cellosaurus CVCL_B668
CLO CLO_0100447
Wikidata Q54950034

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Supernumerary embryos from IVF treatment?
Yes

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
TRA 2-39
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Passage number: 14

Other Genotyping (Cell Line)