FHC.1.H.iPSC.3

General

Cell Line

hPSCreg Name
RIi003-A
Alternative name(s)
FHC.1.H.iPSC.3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MDCi009-A
(8993-C11)
Donor diseases:
Leigh Disease
TIGETi003-A
(GLD1.3)
Donor diseases:
Krabbe disease
UAMi001-A
(PCCA23-FiPS4F8)
Donor diseases:
Propionic Acidemia
BGUi003-A
(BGU03iPOR)
Donor diseases:
P450 Oxidoreductase Deficiency
BGUi001-A
(BGU01iPORhet)
Donor diseases:
P450 Oxidoreductase Deficiency
BGUi002-A
(BGU02iPOR)
Donor diseases:
P450 Oxidoreductase Deficiency
Last update 3rd September 2022
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_C417
CLO CLO_0101531
Wikidata Q54834872

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
familial hypercholesterolemia
The donor is affected.

Ethics

Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
How may genetic information associated with the cell line be accessed?
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM F/12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine 2 mM
β-mercaptoethanol 0.1 mM
nonessential amino acid 1 %
penicillin 100 units/ml
streptomycin 100 µg/ml
insulin-transferrin-selenite
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)