General

Cell Line
hPSCreg name	SCTCi039-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	SCTCi039-A-1
Alternative name(s)	NGC22.3.369
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	13th August 2025
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Provider
Generator	Radboudumc Stem Cell Technology Center (SCTC) Contact: VIB - Center for Molecular Neurology (VIBCMN)
Owner	VIB - Center for Molecular Neurology (VIBCMN)
Distributors	VIB - Center for Molecular Neurology (VIBCMN)
Derivation country	Belgium
External Databases
BioSamples	SAMEA118790079
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	SCTCi039-A

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Developmental and epileptic encephalopathy, 7 The donor is a carrier of a disease-associated mutation and affected. Synonyms DEE7 EIEE7 KCNQ2-NEE KCNQ2-related epileptic encephalopathy KCNQ2-related neonatal epileptic encephalopathy developmental and epileptic encephalopathy 7 epileptic encephalopathy, early infantile, 7 epileptic encephalopathy, early infantile, type 7 KCNQ2-related disorders show more synonyms show less synonyms Genetic variants KCNQ2 (target) Chromosome location 20q13 Nucleotide sequence variant in HGVS format NM_172107.4:c.601C>T Protein sequence variant in HGVS format NP_742105.1:p.Arg201Cys Is the variant homozygous or heterozygous? Heterozygous
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA115428392

Ethics

Also have a look at the ethics information for the parental line SCTCi039-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	No

hIPSC Derivation

General
The source cell information can be found in the parental cell line SCTCi039-A.
Reprogramming method
Vector free reprogramming
Other
Derived under xeno-free conditions	No
Derived under GMP?	Yes
Available as clinical grade?	No

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
CO2 Concentration	5 %
Medium	Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles

Morphology

Morphology pictures

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

Marker	Expressed
EOMES	Yes

Morphology

ISO_R201C_L_Certificate of Analysis_SA.pdf

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

Marker	Expressed
Desmin	Yes

Morphology

ISO_R201C_L_Certificate of Analysis_SA.pdf

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

Marker	Expressed
NESTIN	Yes

Morphology

ISO_R201C_L_Certificate of Analysis_SA.pdf

Microbiology / Virus Screening
Mycoplasma	Negative

Certificate of Analysis
Is there a certificate of analysis available?	Yes ISO_R201C_L_Certificate_of_Analysis_SA.pdf Passage: 26

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	No
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Developmental and epileptic encephalopathy, 7 Synonyms DEE7 EIEE7 KCNQ2-NEE KCNQ2-related epileptic encephalopathy KCNQ2-related neonatal epileptic encephalopathy developmental and epileptic encephalopathy 7 epileptic encephalopathy, early infantile, 7 epileptic encephalopathy, early infantile, type 7 KCNQ2-related disorders show more synonyms show less synonyms Genetic modifications KCNQ2 (target) Type of modification Isogenic modification Chromosome location 20q13 Nucleotide sequence variant in HGVS format NM_172107.4:c.601T>C Protein sequence variant in HGVS format NP_742105.1:p.Cys201Arg Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? Repaired