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General
iPSC Line |
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| hPSCreg name | SCTi003-A-5 |
| Cite as: | SCTi003-A-5 |
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 2nd September 2026 |
| Notes | Genome-edited Human iPSC Line, SCTi003-A-5, SNCA A53T, referred to as SCTi003-A-5, was generated by CRISPR/Cas9 technology, which introduced the SNCA A53T (c.157G>A) missense mutation into the endogenous alpha-synuclein (SNCA) locus. The A53T mutation was the first genetic mutation identified in familial Parkinson’s disease (PD) and is characterized by an increased propensity for alpha-synuclein aggregation into toxic oligomers and fibrils. This mutation is associated with early-onset Parkinsonism, rapid disease progression, and significant neurodegeneration of dopaminergic neurons (Ohgita et al.; Pushmann et al.).
This product’s parental human induced pluripotent stem cell (hiPSC) line, Healthy Control Human iPSC Line, Female, SCTi003-A (Catalog #200-0511) is a well characterized control line derived from peripheral blood mononuclear cells (PBMCs) of a 48-year-old donor. Targeted gene modifications were confirmed by Sanger sequencing. Post-editing, extensive quality control procedures were undertaken in the manufacturing process for SCTi003-A-5 to ensure optimal product performance and reproducibility. SCTi003-A-5 is karyotypically stable, expresses markers of the undifferentiated state, and remains capable of directed differentiation into all three germ layers including neural lineage cells relevant for PD research. This genome-edited hiPSC line and its isogenic parental hiPSC line provide a genetically defined tool for modeling synucleinopathy, investigating protein aggregation mechanisms, and preclinical evaluation of neuroprotective therapeutics. SCTi003-A-5 is manufactured using mTeSR™ Plus (Catalog #100-0276) and is compatible with STEMdiff™ cell culture media, allowing for standardized high-quality maintenance and differentiation to various cell types, such as midbrain neurons, astrocytes, and microglia. Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols. |
| User feedback | |
Provider |
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| Generator |
STEMCELL Technologies Inc. (SCT)
Contact:
STEMCELL Technologies Inc. (SCT) |
| Owner | STEMCELL Technologies Inc. (SCT) |
| Distributors | |
External iPSC Databases |
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| BioSamples | SAMEA123396601 |
General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Additional restrictions:
For in vitro research use only. Not approved for diagnostic, therapeutic, or clinical applications. Not approved for human or veterinary use in vivo. |
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iPSC Derivation
General |
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The source cell information can be found in the parental cell line
SCTi003-A.
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