SKLRMe011-A
General
Cell Line |
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| hPSCreg name | SKLRMe011-A |
| Cite as: | SKLRMe011-A |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 16th October 2024 |
| User feedback | |
Provider |
|
| Generator | State Key Laboratory of Reproductive Medicine, Nanjing Medical University (SKLRM) |
| Owner | State Key Laboratory of Reproductive Medicine, Nanjing Medical University (SKLRM) |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA116085257 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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External Databases (Donor) |
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| BioSamples | SAMEA116085259 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | ethical committee of Nanjing Medical University |
| Approval number | 2012-SR-128 |
hESC Derivation
| Date of derivation | 2023-12-13 |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
Yes |
| Expansion status |
5
|
| ICM morphology |
A
|
| Trophectoderm morphology |
a
|
| ZP removal technique | Manual Removal of ZP |
| Cell seeding | Isolated ICM |
| Derivation under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46，XX
Passage number: 5
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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