The cell line is not validated yet.
SPPHIi005-A
General
Cell Line |
|
| hPSCreg name | SPPHIi005-A |
| Cite as: | SPPHIi005-A (RRID:CVCL_D1G6) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 27th March 2024 |
| User feedback | |
Provider |
|
| Generator | Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital (SPPHI) |
| Owner | Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital (SPPHI) |
| Distributors | |
| Derivation country | China |
External Databases |
|
| Cellosaurus | CVCL_D1G6 |
| BioSamples | SAMEA115443060 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Clinical trials, organoid preparation
Research use: allowed
Additional restrictions:
Prohibited for human experimentation |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA115443061 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | hao429704 |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | The human ethics committee was from the Sichuan Provincial People’s Hospital, the affiliate hospital of the University of Electronic Science and Technology of China |
| Approval number | AF-17/01.0 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Mononuclear cells collected from peripheral blood.
Synonyms
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Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus () |
| Is the used vector excisable? |
Yes |
| Absence of reprogramming vector(s)? |
Yes |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
Immunostaining, PCR, Sequencing
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Essential 8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| SSEA-4 |
Yes |
|
| Marker | Present | Absent |
| mCpG | ||
| OCT4 | X |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
enter
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Other Genotyping (Cell Line) |
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