RES116, Cell line 20 116:5

General

Cell Line

hPSCreg name SUHi021-A
Cite as:
SUHi021-A
Alternative name(s)
RES116, Cell line 20 116:5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SUHi020-A
(RES114, Cell line 19 114:5)
Donor diseases:
Obesity
SUHi022-A
(RES118, Cell line 21 118:5)
Donor diseases:
Obesity
SUHi023-A
(RES121, Cell line 22 121:5)
Donor diseases:
Obesity
Type 2 Diabetes Mellitus
SUHi024-A
(RES174, Cell line 23 174)
Donor diseases:
Obesity
SUHi013-A
(RES110, Cell line 11 110:5)
Donor diseases:
Obesity
SUHi015-A
(RES141, Cell line 15 141)
Donor diseases:
Obesity
SUHi016-A
(RES142, Cell line 16 142:3)
Donor diseases:
Obesity
Last update 26th March 2024
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Provider

Generator Sahlgrenska University Hospital (SUH)

External Databases

BioSamples SAMEA115437230

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Obesity
  • Obesity, unspecified
  • obesity

External Databases (Donor)

BioSamples SAMEA115437235

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The Swedish Ethical Review Authority
Approval number Dnr 2019-04179
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name Human Skin fibroblast cells
Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Passage method: 1)Aspirate mTeSR plus media and wash cells once with 1X PBS. 2)Add 0,02% EDTA in 1X PBS and incubate at room temperature for 2-4 mins. 3)Aspirate 0,02% EDTA in 1X PBS. 4)Resuspend the cells in mTeSR plus media containing 0,01% Penicillin/Streptomycin (P/S). 5)Transfer the cell suspension to a new plate coated with Matrigel containing mTeSR plus media plus 0,01% P/S.
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1P5 /OCT4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
GATA binding protein 4
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
CXCR4
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Pax6
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)