General#

Cell Line
hPSCreg Name A standardised name that was automatically generated based on the cell line information. see Naming Tool	TECHe004-A
Alternative name(s)	J3
Cell line type	Human embryonic stem cell (hESC)
Last update	22nd May 2019
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Provider
Generator	Technion - Israel Institute of Technology (IIT) Contact: Israel Institute of Technology - Regulation of Gene Expression, Development, Differentiation and Cancer
Distributors	European Collection of Authenticated Cell Cultures (ECACC)
Derivation country	Israel
External Databases
Cellosaurus	CVCL_C334
CLO	CLO_0100123
Wikidata	Q54898401
General Information
* Is the cell line readily obtainable for third parties?	No
Subclones	TECHe004-A-1

Donor Information#

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases

Ethics#

Alternatives to consent

hESC Derivation#

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes
SSEA-1	No
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY Passage number: NP
Other Genotyping (Cell Line)