UCSFe003-A-1

HSF-1-14

General#

Cell Line

hPSCreg Name UCSFe003-A-1
Alternative name(s)
HSF-1-14
Cell line type Human embryonic stem cell (hESC)
Last update 22nd November 2017
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Provider

Generator University of California, San Francisco (UCSF)
Derivation country United States

External Databases

Cellosaurus CVCL_D004

General Information

Is the cell line available in principle?
Yes
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)

Genetic Modification#