UCSF-1
UCSFe004-A
General
Cell Line |
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| hPSCreg name | UCSFe004-A |
| Cite as: | UCSFe004-A (RRID:CVCL_A019) |
| Alternative name(s) |
UCSF-1
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
Conklin Lab, Gladstone/UCSF (UCSF)
Contact:
Human Stem Cell Biology |
| Derivation country | United States |
External Databases |
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| Cellosaurus | CVCL_A019 |
| Wikidata | Q54990050 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| ZP removal technique | Chemical |
Culture Conditions
| Feeder cells |
Placenta fibroblasts Cellfinder Ont Id: FMA_63934 |
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| Medium |
Other medium:
Base medium: X-VIVO10
Main protein source: Knock-out serum replacement Serum concentration: % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| TERT |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 17
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Other Genotyping (Cell Line) |
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