UCSF-2

General

Cell Line

hPSCreg name UCSFe005-A
Cite as:
UCSFe005-A (RRID:CVCL_A020)
Alternative name(s)
UCSF-2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Conklin Lab, Gladstone/UCSF (UCSF)
Derivation country United States

External Databases

Cellosaurus CVCL_A020
Wikidata Q54990051

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Chemical

Culture Conditions

Feeder cells Placenta Fibroblasts
Cellfinder Ont Id: FMA_63934
Medium Other medium:
Base medium: X-VIVO10
Main protein source: Knock-out serum replacement
Serum concentration: %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TERT
Yes
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)