UGENTi005-A

EMC368i/c1

The cell line is not validated yet.

General

Cell Line

hPSCreg name UGENTi005-A
Cite as:
UGENTi005-A
Alternative name(s)
EMC368i/c1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 18th June 2025
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Provider

Generator Ghent University (UGENT)
Owner Ghent University (UGENT)
Derivation country Belgium

External Databases

BioSamples SAMEA117842364

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 70-74
Ethnicity Causasian (Belgian)

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
RPE65 (target)
NM_000329.3:c.1555G>A
NP_000320.1:p.(Glu519Lys)
Heterozygous
1481159
https://doi.org/10.21203/rs.3.rs-5849564/v2

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
Genome sequencing

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA117842384

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
* Does consent expressly prevent the derivation of pluripotent stem cells? No
* Does consent pertain to a specific research project? Yes
Details on restriction to research project Title: Solving missing heritability in inherited retina! diseases using integrated omics in human cellular models
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Medical Ethics Committee of Ghent University Hospital
Approval number BC-09345
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Medical Ethics Committee of Ghent University Hospital
Approval number BC-09345
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
Committed, erythroid stem cells derived from myeloid stem cells. The progenitor cells develop in two phases: erythroid burst-forming units (bfu-e) followed by erythroid colony-forming units (cfu-e). Bfu-e differentiate into cfu-e on stimulation by erythropoietin, and then further differentiate into erythroblasts when stimulated by other factors.
Source cell origin
1: The fluid that circulates in the heart, arteries, capillaries, and veins of a vertebrate animal carrying nourishment and oxygen to and bringing away waste products from all parts of the body. 2: A comparable fluid of an invertebrate.
Synonyms
  • whole blood
Source cell type (free text) Culture and expansion of erythroid progenitors from PBMCs
Age of donor (at collection) 70-74
Collected in 2023

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
Immunostaining, RT-PCR
Notes on reprogramming vector detection (A) Vector clearance was assessed by IHC with antibody against SeV (B) Vector clearance was evaluated by RT-PCR for c-Myc, KLF4, KOS and SeV
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
TRA 1-81
Yes
SSEA-4
Yes
Morphology pictures
Morphology iPSC lines
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Morphology
Immunostaining trilineage differentiation markers.
qPCR trilineage differentiation markers
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
KDR
Yes
TBXT
Yes
NCAM1
Yes
Morphology
Immunostaining trilineage differentiation markers
qPCR trilineage differentiation markers
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
SOX1
Yes
TUBB (b-tubulin)
Yes
Morphology
qPCR trilineage differentiation markers
Immunostaining trilineage differentiation markers

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
shallow whole genome sequencing (CNV-Seq)