FES75

General

Cell Line

hPSCreg Name
UHe006-A
Alternative name(s)
FES75
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator University of Helsinki (UH)
Derivation country Finland

External Databases

Cellosaurus CVCL_B137
CLO CLO_0100119
Wikidata Q54834762

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

Surface coating hFF
Feeder cells human foreskin fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Supplements
FGF2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 15

Other Genotyping (Cell Line)