General

Cell Line
hPSCreg name	UIOi002-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	UIOi002-A (RRID:CVCL_C1X2)
Alternative name(s)	iPSC APOE3/3: Unaffected (Coriell #AG09173, female, Age 75)
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	7th April 2022
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Provider
Generator	University of Oslo (UIO) Contact: University of Oslo (UIO)
Owner	Harvard Medical School
Distributors	Harvard Medical School
Derivation country	United States
External Databases
BioSamples	SAMEA14018866
Cellosaurus	CVCL_C1X2
Wikidata	Q114313162
General Information
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed.
Disease associated phenotypes	Alzheimer's disease
Karyotyping (Donor)
Has the donor karyotype been analysed?	Yes Li-Huei_Tsai_MIT-Neuron_APOE42018.pdf
External Databases (Donor)
BioSamples	SAMEA14018867

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	No
Please provide contact information of the holder of the original Donor Information Sheet.	https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AG10788&Product=CC
Do you (Depositor/Provider) hold the original Donor Consent Form?	No
If you do not hold the Donor Consent Form, do you know who does?	No
Alternatives to consent are available?	No
Is there other documentation provided to the donor for consenting purposes?	No
Confirm that consent was obtained by a qualified professional	Yes
Has the donor agreed to be re-contacted?	Unknown
Has the donor been informed about how her/his data will be protected?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	No
Does consent expressly prevent the derivation of pluripotent stem cells?	Yes
Does consent pertain to a specific research project?	No
Does consent permit unforeseen future research, without further consent?	Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications?	No
Does consent expressly prevent development of commercial products?	Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it?	Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time?	Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent permit research by
an academic institution?	Yes
a public organisation?	Yes
a non-profit company?	Yes
a for-profit corporation?	Yes
Does consent expressly permit collection of genetic information?	Yes
Does consent expressly permit storage of genetic information?	Yes
Does consent prevent dissemination of genetic information?	Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens?	Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor?	Yes
How may genetic information associated with the cell line be accessed?	No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells?	No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples?	Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor?	Yes
Does consent permit access to medical records of the donor?	No
Does consent permit access to any other source of information about the clinical treatment or health of the donor?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells?	No
Do you have obligations to third parties in regard to the use of the cell line?	No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells?	No
Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	No

hIPSC Derivation

General
Source cell type	Fibroblasts
Reprogramming method
Vector type	Integrating
Vector	Detailed information on methods to generate and culture the cells was in the paper https://pubmed.ncbi.nlm.nih.gov/29861287/.
Is the used vector excisable?	Unknown
Absence of reprogramming vector(s)?	Unknown
Reprogramming vectors silenced?
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Medium

Other medium: Base medium: Detailed information on methods to generate and culture the cells was in the paper https://pubmed.ncbi.nlm.nih.gov/29861287/. Main protein source: Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles

EpiPluriScore

Score:

Marker	Present	Absent
mCpG
OCT4

Report

Li-Huei Tsai MIT-Neuron APOE42018.pdf

Morphology

Morphology pictures

Li-Huei Tsai MIT-Neuron APOE42018.pdf

Other pluripotency tests used

Detailed information on methods to generate and culture the cells was in the paper https://pubmed.ncbi.nlm.nih.gov/29861287/.

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Unknown
Other Genotyping (Cell Line)