iLB-C-108bm-s3

General#

Cell Line

hPSCreg Name UKBi012-A
Alternative name(s)
iLB-C-108bm-s3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Universitätsklinikum Bonn (UKB)
Owner Universitätsklinikum Bonn
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA104382074
Cellosaurus CVCL_LE35

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasia, German

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104382075

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 30-34
Collected in 2015
Passage number reprogrammed d9

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-81
Yes
TRA 1-60
Yes
Differentiation Potency
Hematopoietic Precursor Cell
Ont Id: CL_0008001
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)