iLB-BAG3-301bm-s2 BAG3 edit
UKBi022-A-1
General
Cell Line |
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| hPSCreg name | UKBi022-A-1 |
| Cite as: | UKBi022-A-1 (RRID:CVCL_E8WI) |
| Alternative name(s) |
iLB-BAG3-301bm-s2 BAG3 edit
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 16th October 2024 |
| User feedback | |
Provider |
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| Generator |
Universitätsklinikum Bonn (UKB)
Contact:
Institute of Physiology I (PHYSI) |
| Owner | Institute of Physiology I (PHYSI) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA116131058 |
| Cellosaurus | CVCL_E8WI |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | N/A |
| Is the medical history available upon request? | N/A |
| Is clinical information available? | N/A |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA116110574 |
Ethics
Also have a look at the ethics information for the parental line
UKBi022-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UKBi022-A.
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| Passage number reprogrammed | p9 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Other medium:
Base medium: StemMACS iPS Brew XF Medium
Main protein source: Serum concentration: % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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