UKERi33Q-R2-006

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
UKERi009-A
Alternative name(s)
UKERi33Q-R2-006
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th April 2022
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Provider

Generator Universitätsklinikum Erlangen (UKER)
Owner Universitätsklinikum Erlangen (UKER)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA13024890
CLO CLO_0103747
Cellosaurus CVCL_C1JQ
Wikidata Q114313170

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX

External Databases (Donor)

BioSamples SAMEA13024891

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethics Committee Friedrich-Alexander University Erlangen-Nürnberg
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
fibroblast of dermis
Source cell origin
Arm Skin
The skin or integument covering the arm.
Synonyms
  • ARM SKIN
  • Arm Skin
Age of donor (at collection) 45-49

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Feeder cells
No
Passage method Enzymatically
CO2 Concentration 5 %
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Hematopoietic Precursor Cell
Ont Id: CL_0008001
In vitro directed differentiation
Marker Expressed
IBA1
Yes
PTPRC
Yes
SPN
Yes
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
DCX
Unknown
SOX2
Unknown

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 5
Karyotyping method: G-Banding

Other Genotyping (Cell Line)