NP0105-19

General#

Cell Line

hPSCreg Name UKKi021-B
Alternative name(s)
NP0105-19
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 26th July 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Klinikum der Universität zu Köln (UKK)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA103988346
Cellosaurus CVCL_LD32
ECACC 66540565
EBiSC UKKi021-B

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity indian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885569

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 30-34
Collected in 2016
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection episomal vector cassette absent
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex Basal Medium
Main protein source:
Serum concentration: %
Supplements
StemFlex Supplement (10x) 50 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT 4
Yes
SOX2
Yes
SSEA-1
Yes
SSEA-4
Yes
TRA 1-80
Yes
CD90
Yes
CD30
Yes
Score: 0.24
Marker Present Absent
mCpG
OCT4
Morphology pictures
hiPS NP0105-19 before banking and after thawing
hiPS NP0105-19 on different days post thaw
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Chr07: Larger duplication (20Mbp) mosaic (80%) on long arm.
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)