NCL-2
UNEWe002-A
General
Cell Line |
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| hPSCreg name | UNEWe002-A |
| Cite as: | UNEWe002-A (RRID:CVCL_A003) |
| Alternative name(s) |
NCL-2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
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| Generator | University of Newcastle (UNEW) |
| Derivation country | United Kingdom |
External Databases |
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| Cellosaurus | CVCL_A003 |
| Wikidata | Q54908227 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Was the embryo established purely for research purposes? | No |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent | |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
| Date of derivation | 2004-10-01 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | Immunosurgery |
| Cell seeding | Embryo |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 10 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| TERT |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46 XX
Passage number: 20
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Other Genotyping (Cell Line) |
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