NCL-5
UNEWe005-A
General
iPSC Line |
|
| hPSCreg name | UNEWe005-A |
| Cite as: | UNEWe005-A (RRID:CVCL_A006) |
| Alternative name(s) |
NCL-5
|
| iPSC line type | Human embryonic stem cell (hESC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
|
| Generator | University of Newcastle (UNEW) |
| Derivation country | United Kingdom |
External iPSC Databases |
|
| Cellosaurus | CVCL_A006 |
| Wikidata | Q54908230 |
General iPSC Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
iPSC Culture Conditions
| Surface coating | Gelatin | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| TERT |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
46 XX
Passage number: 20
|
Other Genotyping (iPSC Line) |
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