UNEW011Ai

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name UNEWi011-A
Alternative name(s)
UNEW011Ai
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th January 2018
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Provider

Generator University of Newcastle (UNEW)
Distributors

External Databases

BioSamples SAMEA2629624
EBiSC UNEWi011-A
ECACC 66540041
Cellosaurus CVCL_VF32

General Information

Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
XLF Deficiency
The donor is affected.
Stage
Severe
Non-disease associated phenotypes
  • primary immunodeficiency, severe growth retardation, bird-like face, recurrent perianal abscess, pancytopenia, and polydactyly
Family history NO

External Databases (Donor)

BioSamples SAMEA2629494

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 1-4

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 95 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

No characterisation data could be found.

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unkown

Other Genotyping (Cell Line)