F018 13.2

General#

Cell Line

hPSCreg Name UNEWi021-A
Alternative name(s)
F018 13.2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA4092799
Cellosaurus CVCL_IT82
ECACC 66540174
EBiSC UNEWi021-A

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4092787

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 80-84
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Based on morphology, growth rate and expression of SSEA4 and NANOG
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
Retinal Pigment Epithelium
Ont Id: BTO_0001177
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
Tuj1
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: P10

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)