F343 clone 3

General#

Cell Line

hPSCreg Name UNEWi023-B
Alternative name(s)
F343 clone 3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA4567587
Cellosaurus CVCL_IT89
ECACC 66540442
EBiSC UNEWi023-B

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
age-related macular degeneration
Synonyms
  • Macular Dystrophy
  • Degeneration, Macular
  • Age Related Maculopathies
  • MACULAR DEGENERATION NOS
  • Age related maculopathy
  • Age Related Macular Degeneration
  • Unspecified senile macular degeneration
  • Maculopathies, Age Related
  • MACULOPATHY AGE RELAT
  • AAMD - Age related macular degeneration
  • Maculopathies, Age-Related
  • Macular Dystrophies
  • ARMD
  • Amended
  • Dystrophies, Macular
  • Age-Related Maculopathy
  • Senile macular degeneration
  • SMD - Senile macular degeneration
  • Macular degeneration (senile) of retina, unspecified
  • Macular Degeneration, Age-Related
  • Degeneration, Age-Related Macular
  • Macular degeneration (disorder)
  • Macular Degenerations
  • Degenerations, Age-Related Macular
  • AGE RELAT MACULOPATHIES
  • Macular Degenerations, Age-Related
  • Maculopathy, Age-Related
  • AMD - Age-related macular degeneration
  • Maculopathy, Age Related
  • Dystrophy, Macular
  • Degenerations, Macular
  • Senile macular retinal degeneration
  • Age-related macular degeneration (disorder)
  • Age related macular degeneration (disorder) [Ambiguous]
  • AGE RELAT MACULOPATHY
  • MACULOPATHIES AGE RELAT
  • Age-Related Macular Degenerations
  • Age-Related Maculopathies
  • Senile macular degeneration of retina
  • ARMD - Age-related macular degeneration
  • Macular Degeneration
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4567586

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 85-89
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Based on morphology, growth rate and expression of SSEA4 and NANOG
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
Alpha FetoProtein
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
TUJ-1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: P9

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)