UOSe012-A-1
General
Cell Line |
|
| hPSCreg name | UOSe012-A-1 |
| Cite as: | UOSe012-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
UOSe012-A (MstrShef7, MShef7, MasterShef7) |
| Last update | 4th August 2023 |
| User feedback | |
Provider |
|
| Generator |
University of Southampton (SOTON)
Contact:
University of Southampton (SOTON) |
| Owner | University of Southampton (SOTON) |
| Derivation country | United Kingdom |
External Databases |
|
| BioSamples | SAMEA114236725 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
Cell available for collaboration purposes and subject to user approval by UK Stem Cell Bank. |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA104627893 |
Ethics
Also have a look at the ethics information for the parental line
UOSe012-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
UOSe012-A.
|
Culture Conditions
| Surface coating | Laminin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Nutristem hPSC XF
Main protein source: Albumine Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro directed differentiation
Morphology
RL21GFP cone.tif
RL2.1GFP, day 144, retinal organoid
merge set 1.tif
CRX (red), RCVRN (green) immuno staining d44, retinal organoid
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Unknown
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
|
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