Regea 07/027
UTAe003-A
General
Cell Line |
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| hPSCreg name | UTAe003-A |
| Cite as: | UTAe003-A (RRID:CVCL_B356) |
| Alternative name(s) |
Regea 07/027
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
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| Generator |
University of Tampere (UTA)
Contact:
Stem Cell Research Group |
| Derivation country | Finland |
External Databases |
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| Cellosaurus | CVCL_B356 |
| Wikidata | Q54949705 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Regional ethical committee |
hESC Derivation
| Date of derivation | 2007-05-15 |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
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| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 23
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Other Genotyping (Cell Line) |
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