VUBe001-A-1
General
Cell Line |
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| hPSCreg name | VUBe001-A-1 |
| Cite as: | VUBe001-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
VUBe001-A (VUB01) |
| Last update | 15th October 2021 |
| User feedback | |
Provider |
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| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
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General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
VUBe001-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
VUBe001-A.
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Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040 |
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 10 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| LIN28 |
Yes |
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| NANOG |
Yes |
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| NPM1 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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