NCATS-CL4510
The cell line is not validated yet.
WTSIi018-B-37
General
Cell Line |
|
| hPSCreg name | WTSIi018-B-37 |
| Cite as: | WTSIi018-B-37 |
| Alternative name(s) |
NCATS-CL4510
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 20th May 2026 |
| User feedback | |
Provider |
|
| Generator | NIH/NCATS-TRND Branch (TRND) |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Genetic Modification
| Disease/phenotype related modifications |
|
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
|
Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has son:
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External Databases (Donor) |
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| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
Ethics
Also have a look at the ethics information for the parental line
WTSIi018-B
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
WTSIi018-B.
|
|
Reprogramming method |
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Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| Medium |
Other medium:
Base medium: StemFlex™
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY
Passage number: 29
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
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