H240815 48-2, SAMEA4455507

General#

Cell Line

hPSCreg Name BIONi031-A
Alternative name(s)
H240815 48-2, SAMEA4455507
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BSC)
Owner Bioneer (BSC)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA4455507
Cellosaurus CVCL_LE29
ECACC 66540404
EBiSC BIONi031-A

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Non-disease associated phenotypes
  • Low Birth Weight

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4455506

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
adipose tissue derived mesenchymal stem cell
A adult mesenchymal stem cell derived from adipose tisssue
Synonyms
  • human mesenchymal stem cell from adipose tissue
  • hMSC-AT
Source cell origin
adipose tissue
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
  • fat
  • fatty tissue
  • fat tissue
Source cell type (free text) Pre-Adipocyte
Age of donor (at collection) 20-24
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-81
Yes
TDG1
Yes
GDF3
Yes
GABRB3
Yes
DMNT3B
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CD184
Yes
Sox17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CD34
Yes
CD56
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Pax6
Yes
Nestin
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY, normal male karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)