H290715, SAMEA4563391
BIONi034-A
General#
Cell Line |
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| hPSCreg Name | BIONi034-A |
| Alternative name(s) |
H290715, SAMEA4563391
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th December 2017 |
| User feedback | |
Provider |
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| Generator |
Bioneer (BION)
Contact:
Bioneer (BSC) |
| Owner | Bioneer (BSC) |
| Distributors | |
| Derivation country | Denmark |
External Databases |
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| BioSamples | SAMEA4563391 |
| Cellosaurus | CVCL_LE32 |
| ECACC | 66540424 |
| EBiSC | BIONi034-A |
General Information |
|
| Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Non-disease associated phenotypes |
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA4563392 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
| Source cell type |
adipose tissue derived mesenchymal stem cellA adult mesenchymal stem cell derived from adipose tisssue
Synonyms
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| Source cell origin |
adipose tissuePortion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
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| Source cell type (free text) | Pre-Adipocyte |
| Age of donor (at collection) | 20-24 |
| Passage number reprogrammed | 3 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Vector map | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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| TDG1 |
Yes |
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| GDF3 |
Yes |
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| GABRB3 |
Yes |
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| DMNT3B |
Yes |
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Morphology pictures
Differentiation Potency
In vitro directed differentiation
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY, normal male karyotype
|
Other Genotyping (Cell Line) |
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