H290715, SAMEA4563391
BIONi034-A
General#
Cell Line |
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hPSCreg Name | BIONi034-A |
Alternative name(s) |
H290715, SAMEA4563391
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator |
Bioneer (BION)
Contact:
Bioneer (BSC) |
Owner | Bioneer (BSC) |
Distributors | |
Derivation country | Denmark |
External Databases |
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BioSamples | SAMEA4563391 |
Cellosaurus | CVCL_LE32 |
ECACC | 66540424 |
EBiSC | BIONi034-A |
General Information |
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Projects | |
Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Non-disease associated phenotypes |
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA4563392 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
adipose tissue derived mesenchymal stem cellA adult mesenchymal stem cell derived from adipose tisssue
Synonyms
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Source cell origin |
adipose tissuePortion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
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Source cell type (free text) | Pre-Adipocyte |
Age of donor (at collection) | 20-24 |
Passage number reprogrammed | 3 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector map | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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SSEA-1 |
No |
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SSEA-4 |
Yes |
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TRA 1-81 |
Yes |
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TDG1 |
Yes |
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GDF3 |
Yes |
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GABRB3 |
Yes |
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DMNT3B |
Yes |
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Differentiation Potency
In vitro directed differentiation
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XY, normal male karyotype
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Other Genotyping (Cell Line) |
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