H290715, SAMEA4563391
BIONi034-A
General#
Cell Line |
|
| hPSCreg Name | BIONi034-A |
| Alternative name(s) |
H290715, SAMEA4563391
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th December 2017 |
| User feedback | |
Provider |
|
| Generator |
Bioneer (BION)
Contact:
Bioneer (BSC) |
| Owner | Bioneer (BSC) |
| Distributors | |
| Derivation country | Denmark |
External Databases |
|
| BioSamples | SAMEA4563391 |
| Cellosaurus | CVCL_LE32 |
| ECACC | 66540424 |
| EBiSC | BIONi034-A |
General Information |
|
| Projects | |
| Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Non-disease associated phenotypes |
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA4563392 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
| Source cell type |
adipose tissue derived mesenchymal stem cellA adult mesenchymal stem cell derived from adipose tisssue
Synonyms
|
| Source cell origin |
adipose tissuePortion of connective tissue composed of adipocytes enmeshed in areolar tissue
Synonyms
|
| Source cell type (free text) | Pre-Adipocyte |
| Age of donor (at collection) | 20-24 |
| Passage number reprogrammed | 3 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Vector map | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|||||
| NANOG |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| TDG1 |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| DMNT3B |
Yes |
|
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY, normal male karyotype
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.