iFCI022 Clone 4
CRICKi017-A
General
Cell Line |
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hPSCreg name | CRICKi017-A |
Cite as: | CRICKi017-A (RRID:CVCL_D0DC) |
Alternative name(s) |
iFCI022 Clone 4
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
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Last update | 4th April 2024 |
User feedback | |
Provider |
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Generator | The Francis Crick Institute Limited (CRICK) |
Owner | The Francis Crick Institute Limited (CRICK) |
Distributors | |
Derivation country | United Kingdom |
External Databases |
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Cellosaurus | CVCL_D0DC |
Wikidata | Q123030966 |
BioSamples | SAMEA115470504 |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA115470505 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
If you do not hold the Donor Consent Form, do you know who does? | Yes |
Please provide the contact information | http://www.ctc.ucl.ac.uk |
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | No |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | Open Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | CLINICALTRIALS.GOV |
Approval number | NCT01888601 |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | Cancer Research (CR UK reference below) |
Approval number | C11496/A17786 |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell type | |
Reprogramming method |
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Vector type | None |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
mRNA
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mRNA | |
Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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SSEA-1 |
No |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
Karyostat BY THERMO
Passage number: 8
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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