IDP52555 #44

General

Cell Line

hPSCreg Name
CRTDi011-D
Alternative name(s)
IDP52555 #44
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th June 2022
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Provider

Generator Center for Regenerative Therapies Dresden (CRTD)
Owner Center for Regenerative Therapies Dresden (CRTD)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA110177241
CLO CLO_0103898
Cellosaurus CVCL_C1PB

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA110187716

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Dr. Nataliya Di Donato, Leiterin Forschungsgruppe - Hirnfehlbildungen, Leiterin Genetische Ambulanz, University Hospital Dresden
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? No
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethikkommission Technische Universität Dresden
Approval number SR-EK-237052022
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethikkommission Technische Universität Dresden
Approval number SR-EK-237052022
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Thermo Fisher Scientific
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Collected in 2021
Passage number reprogrammed P1

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
IDP52555 #44 Endo DAPI+SOX17-A488_20x merge.tif
IDP52555 #44 hiPSCs differentiated to Endoderm in Monolayer and stained with SOX17-Alexa488 antibody and DAPI (merged picture)
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
Morphology
IDP52555 #44 Meso DAPI+BRACHYURY-APC_20x merge.tif
IDP52555 #44 hiPSCs differentiated to Mesoderm in Monolayer and stained with BRACHYURY/TBXT-APC antibody and DAPI (merged picture)
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
PAX6
Yes
NES
Yes
TUBB3 (TUJ1)
Yes
Morphology
IDP52555 #44 EBs DAPI+TUJ1-A488_20x merge.tif
IDP52555 #44 hiPSCs differentiated to Embryoid Bodies and stained with TUJ1-Alexa488 antibody and DAPI (merged picture)
IDP52555 #44 hiPSCs differentiated to Ectoderm in Monolayer and stained with DAPI
IDP52555 #44 Ecto DAPI+A488+A647_20x merge.tif
IDP52555 #44 hiPSCs differentiated to Ectoderm in Monolayer and stained with DAPI, NESTIN-Alexa488 and PAX6-Alexa647 antibody (merged picture)
IDP52555 #44 hiPSCs differentiated to Ectoderm in Monolayer and stained with NESTIN-Alexa488 antibody
IDP52555 #44 hiPSCs differentiated to Ectoderm in Monolayer and stained with PAX6-Alexa647 antibody

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX[cp20]
Passage number: P16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)