HVRDe001-A-1
General
iPSC Line |
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| hPSCreg name | HVRDe001-A-1 |
| Cite as: | HVRDe001-A-1 |
| iPSC line type | Human embryonic stem cell (hESC) |
| Similar iPSC lines |
HVRDe001-A (HuES1) |
| Last update | 24th August 2017 |
| User feedback | |
Provider |
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| Generator |
Harvard University (HVRD)
Contact:
Melton Lab (MLB) |
| Derivation country | United States |
External iPSC Databases |
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General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
iPSC Genetic Modification
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
HVRDe001-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
HVRDe001-A.
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iPSC Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040 |
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| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 10 % Supplements
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iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
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Other Genotyping (iPSC Line) |
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