HS491
KIe028-A
General
Cell Line |
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| hPSCreg name | KIe028-A |
| Cite as: | KIe028-A (RRID:CVCL_C226) |
| Alternative name(s) |
HS491
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 11th April 2019 |
| User feedback | |
Provider |
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| Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
| Derivation country | Sweden |
External Databases |
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| Cellosaurus | CVCL_C226 |
| Wikidata | Q54896334 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Regional ethical committee |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
No |
| ZP removal technique | Mechanical |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
Human Foreskin Cellfinder Ont Id: CRL-2429, ATCC, Manassas, VA |
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
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