HS539
KIe031-A
General
Cell Line |
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| hPSCreg Name | KIe031-A |
| Alternative name(s) |
HS539
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 3rd September 2022 |
| User feedback | |
Provider |
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| Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
| Derivation country | Sweden |
External Databases |
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| Cellosaurus | CVCL_C227 |
| CLO | CLO_0100493 |
| Wikidata | Q54896336 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
| Disease associated phenotypes |
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Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Regional ethical committee/ Regional ethics board, Stockholm |
| Approval number |
hESC Derivation
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
Yes |
Culture Conditions
| Feeder cells |
Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
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