HS539

General#

Cell Line

hPSCreg Name KIe031-A
Alternative name(s)
HS539
Cell line type Human embryonic stem cell (hESC)
Last update 11th April 2019
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Provider

Generator Karolinska Institutet (KI)
Derivation country Sweden

External Databases

Cellosaurus CVCL_C227

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases
Disease associated phenotypes
  • monogenetic disease

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regional ethical committee/ Regional ethics board, Stockholm

hESC Derivation#

Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Feeder cells
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)