The cell line is not validated yet.
KUCFRi005-A
General
Cell Line |
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| hPSCreg name | KUCFRi005-A |
| Cite as: | KUCFRi005-A (RRID:CVCL_C1U1) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 12th August 2022 |
| User feedback | |
Provider |
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| Generator | Korea University Cell Function Regulation Lab (KUCFR) |
| Owner | Korea University Cell Function Regulation Lab (KUCFR) |
| Distributors | |
| Derivation country | South Korea |
External Databases |
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| BioSamples | SAMEA110644817 |
| Cellosaurus | CVCL_C1U1 |
| Wikidata | Q114311863 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
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External Databases (Donor) |
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| BioSamples | SAMEA110644819 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | No |
| Was the consent voluntarily given? | No |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | Original line (fibroblast) has been obtained by Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration. |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | Original line (fibroblast) has been obtained by Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration. |
| Alternatives to consent are available? | No |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | No |
| Has the donor agreed to be re-contacted? | No |
| Has the donor been informed about how her/his data will be protected? | No |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type | |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Unknown |
| Methods used |
Immunostaining
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Other medium:
Base medium: equal DMEM/F12 (Thermo Fisher Scientific) and Neurobasal (Thermo Fisher Scientific), supplemented with 1% N2 supplement (Thermo Fisher Scientific), 2% B27 supplement (Thermo Fisher Scientific), 1% L-glutamine, 1% nonessential amino acids, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, 5% knockout serum replacement (KSR; Thermo Fisher Scientific)
Main protein source: Knock-out serum replacement Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| NANOG |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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