KUCFRi005-A

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
KUCFRi005-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th August 2022
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Provider

Generator Korea University Cell Function Regulation Lab (KUCFR)
Owner Korea University Cell Function Regulation Lab (KUCFR)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA110644817
CLO CLO_0103974

General Information

* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA110644819

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? No
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Original line (fibroblast) has been obtained by Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration.
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Original line (fibroblast) has been obtained by Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration.
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional No
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
embryonic fibroblast

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown
Methods used
Immunostaining

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: equal DMEM/F12 (Thermo Fisher Scientific) and Neurobasal (Thermo Fisher Scientific), supplemented with 1% N2 supplement (Thermo Fisher Scientific), 2% B27 supplement (Thermo Fisher Scientific), 1% L-glutamine, 1% nonessential amino acids, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, 5% knockout serum replacement (KSR; Thermo Fisher Scientific)
Main protein source: Knock-out serum replacement
Serum concentration: 0 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
36+XX
Passage number: 10

Other Genotyping (Cell Line)