KUCFRi005-A-1

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
KUCFRi005-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th August 2022
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Provider

Generator Korea University Cell Function Regulation Lab (KUCFR)

External Databases

BioSamples SAMEA110644876
CLO CLO_0103975

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA110644819

Ethics

Also have a look at the ethics information for the parental line KUCFRi005-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line KUCFRi005-A.
Passage number reprogrammed 15

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: equal DMEM/F12 (Thermo Fisher Scientific) and Neurobasal (Thermo Fisher Scientific), supplemented with 1% N2 supplement (Thermo Fisher Scientific), 2% B27 supplement (Thermo Fisher Scientific), 1% L-glutamine, 1% nonessential amino acids, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, 5% knockout serum replacement (KSR; Thermo Fisher Scientific)
Main protein source: Knock-out serum replacement
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
36+XX
Passage number: 15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
African Swine Fever Virus
Genetic modifications
CD2 molecule (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System