The cell line is not validated yet.
KUCFRi005-A-1
General
Cell Line |
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| hPSCreg name | KUCFRi005-A-1 |
| Cite as: | KUCFRi005-A-1 (RRID:CVCL_C1U2) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 12th August 2022 |
| User feedback | |
Provider |
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| Generator | Korea University Cell Function Regulation Lab (KUCFR) |
External Databases |
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| BioSamples | SAMEA110644876 |
| Cellosaurus | CVCL_C1U2 |
| Wikidata | Q114311864 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA110644819 |
Ethics
Also have a look at the ethics information for the parental line
KUCFRi005-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KUCFRi005-A.
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| Passage number reprogrammed | 15 |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Unknown |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Other medium:
Base medium: equal DMEM/F12 (Thermo Fisher Scientific) and Neurobasal (Thermo Fisher Scientific), supplemented with 1% N2 supplement (Thermo Fisher Scientific), 2% B27 supplement (Thermo Fisher Scientific), 1% L-glutamine, 1% nonessential amino acids, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, 5% knockout serum replacement (KSR; Thermo Fisher Scientific)
Main protein source: Knock-out serum replacement Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| SSEA-4 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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