LVIP07-SD1-CS1
The cell line is not submitted yet.
(only basic data is shown)
General
Cell Line |
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| hPSCreg name | LVPEIi001-C-1 |
| Cite as: | LVPEIi001-C-1 |
| Alternative name(s) |
LVIP07-SD1-CS1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 26th February 2025 |
| Notes | This is an isogenic, homozygous mutant iPSC line (LVPEIi001-C-1), generated using an exon 44 targeting sgRNA and an engineered en1FnCas9 encoded CRISPR plasmid. It carries a 12-base pair deletion in exon 44 of the ABCA4 gene, which was confirmed by target-region specific PCR amplification of genomic DNA followed by Sanger sequencing. The in-frame deletion results in the loss of 4 amino acids within the second nucleotide-binding domain (NBD2) of ABCA4, which causes structural defects that could impair the protein function. The isogenic line was clonally expanded beyond passage 20. This clonal line maintained the expression of major stemness markers (OCT4, SOX2, NANOG, KLF4, and SSEA4), demonstrated the ability to differentiate into all three germ layers and exhibited a normal male karyotype. |
| User feedback | |
Provider |
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| Generator |
L.V. Prasad Eye Institute (LVPEI)
Contact:
L.V. Prasad Eye Institute (LVPEI) |
| Owner | L.V. Prasad Eye Institute (LVPEI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA117637585 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LVPEIi001-C.
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