LVIP07-SD1-CS1, F2-CTS-ABCA4-CS1, Clone 19
The cell line is not submitted yet.
(only basic data is shown)
General
Cell Line |
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| hPSCreg name | LVPEIi001-C-1 |
| Cite as: | LVPEIi001-C-1 |
| Alternative name(s) |
LVIP07-SD1-CS1, F2-CTS-ABCA4-CS1, Clone 19
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 30th August 2025 |
| Notes | This is an isogenic, ABCA4 gene edited mutant iPSC line derived from the healthy control iPSC line, LVPEIi001-C. It was generated using an exon 44 targeting sgRNA and an engineered en1FnCas9 encoded CRISPR construct. It carries a 12-base pair deletion within the exon 44 of both the alleles of ABCA4 gene. This homozygous genomic edit was confirmed by target-region specific PCR amplification and Sanger sequencing of the genomic DNA. The in-frame deletion resulted in the loss of 4 amino acids within the second nucleotide-binding domain 2 (NBD2) of ABCA4, which causes structural defects that could impair the protein function. The isogenic line was clonally expanded beyond passage 20. This clonal line maintained the expression of major stemness markers (OCT4, SOX2, NANOG, KLF4, SSEA4), demonstrated the ability to differentiate into all three germ layers and exhibited a normal female karyotype. |
| User feedback | |
Provider |
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| Generator |
L.V. Prasad Eye Institute (LVPEI)
Contact:
L.V. Prasad Eye Institute (LVPEI) |
| Owner | L.V. Prasad Eye Institute (LVPEI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA117637585 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LVPEIi001-C.
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