LVIP07-SD1-CS5
The cell line is not submitted yet.
(only basic data is shown)
General
Cell Line |
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| hPSCreg name | LVPEIi001-C-1 |
| Cite as: | LVPEIi001-C-1 |
| Alternative name(s) |
LVIP07-SD1-CS5
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 4th February 2025 |
| Notes | This was an isogenic, homozygous iPSC knockout line (LVPEIi001-C-1), generated using a high-fidelity engineered en1FnCas9 vector. The line carried a 12-base pair deletion in exon 44 of the ABCA4 gene, which was confirmed through genotyping. The 12-base pair deletion resulted in structural defects in the second nucleotide-binding domain (NBD2) of the ABCA4 protein, impairing its function. The isogenic line was clonally expanded beyond passage 20 and maintained expression of major stemness markers (OCT4, SOX2, NANOG, KLF4, and SSEA4). It also demonstrated the ability to differentiate into all three germ layers and exhibited a normal karyotype. |
| User feedback | |
Provider |
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| Generator |
L.V. Prasad Eye Institute (LVPEI)
Contact:
L.V. Prasad Eye Institute (LVPEI) |
| Owner | L.V. Prasad Eye Institute (LVPEI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA117637585 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LVPEIi001-C.
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