iPSC C1-15

General

Cell Line

hPSCreg name NUIGi055-A
Cite as:
NUIGi055-A (RRID:CVCL_A4DC)
Alternative name(s)
iPSC C1-15
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
NUIGi055-B
(iPSC C1-16)
NUIGi055-C
(iPSC C1-17)
Last update 5th January 2021
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Provider

Generator National University of Ireland Galway (NUIG)
Owner National University of Ireland Galway (NUIG)
Distributors
Derivation country Ireland

External Databases

BioSamples SAMEA7601555
Cellosaurus CVCL_A4DC
Wikidata Q105510458

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7601556

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Approval number C.A. 750
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Approval number C.A. 750
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name SAMEA7601555
Source cell type
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Other

Selection criteria for clones clones were randomly selected for characterization.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
TRA 1-60
Yes
Embryoid body formation assay was performed to examine the functional pluripotency of iPSC C1-15. Alpha-smooth muscle (ASM) actin was used as the marker of mesoderm; nestin was used for detection of neuroectoderm; alpha-fetoprotein (AFP) was applied for targeting endoderm lineages.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
Alpha Fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
alpha smooth muscle actin
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Nestin
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)