iPSC C1-16

General#

Cell Line

hPSCreg Name
NUIGi055-B
Alternative name(s)
iPSC C1-16
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th January 2021
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Provider

Generator National University of Ireland Galway (NUIG)
Owner National University of Ireland Galway (NUIG)
Distributors
Derivation country Ireland

External Databases

BioSamples SAMEA7601553
CLO CLO_0103158
Cellosaurus CVCL_A4DD
Wikidata Q105510460

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7601556

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Approval number C.A.750
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Clinical Research Ethics Committee, Merlin Park Hospital, Galway, Ireland
Approval number C.A.750
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
dermal fibroblast
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
Alpha Fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
alpha smooth muscle actin
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Nestin
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Passage number: 15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)