OD005-s9
PFIZi016-A
General
Cell Line |
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| hPSCreg name | PFIZi016-A |
| Cite as: | PFIZi016-A (RRID:CVCL_IJ03) |
| Alternative name(s) |
OD005-s9
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 10th March 2020 |
| User feedback | |
Provider |
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| Generator | Pfizer Limited - Pfizer (PFIZ) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA4454950 |
| Cellosaurus | CVCL_IJ03 |
| Wikidata | Q54947271 |
General Information |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 15-19 |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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External Databases (Donor) |
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| BioSamples | SAMEA4454949 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | Dr Orrin Devinsky |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent pertain to a specific research project? | Yes |
| Details on restriction to research project | Dravet Syndrome / Epilepsy |
| Does consent permit unforeseen future research, without further consent? | Yes |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent expressly prevent development of commercial products? | No |
| Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | Yes |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | Yes |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | NYU Institutional Review Board |
| Approval number | i14-01725 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | NYU Institutional Review Board |
| Approval number | i14-01725 |
| Do you have obligations to third parties in regard to the use of the cell line? | Yes |
| Please describe: | Restricted to research into Dravet Syndrome/Epilepsy |
| Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | Yes |
| Further constraints on use | Restricted to research into Dravet Syndrome/Epilepsy |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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| Source cell type |
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
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| Source cell type (free text) | Blood - PBMC erythroblasts |
| Age of donor (at collection) | 15-19 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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Self-renewal
Negative
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Unknown
Scorecard
Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
20
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
No autosomal or sex chromosome aneuplodies were detected
Passage number: 20
Karyotyping method:
BoBs
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Other Genotyping (Cell Line) |
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