OD001-s7

General#

Cell Line

hPSCreg Name PFIZi021-A
Alternative name(s)
OD001-s7
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd January 2018
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Provider

Generator Pfizer Limited - Pfizer (PFIZ)
Distributors

External Databases

BioSamples SAMEA4563388
ECACC 66540422
Cellosaurus CVCL_RF99

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Dravet syndrome
The donor is affected.
Synonyms
  • SMEI
  • Severe myoclonus epilepsy of infancy
  • Severe myoclonic epilepsy of infancy
  • DS
show more synonyms
Genetic variants
SCN1a (target)
p.T1721K, c.5162 C>A De novo
Disease associated phenotypes
  • GTC seizure
  • Developemental delay
  • Normal station and wide based, shuffled gait

External Databases (Donor)

BioSamples SAMEA4563387

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
erythroblast
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
  • normoblast
Age of donor (at collection) 10-14

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Scorecard
Mesoderm
Ont Id: UBERON_0000926
Scorecard
Ectoderm
Ont Id: UBERON_0000924
Scorecard

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
NO autosomal or sex chromosome aneuploidies were detected
Karyotyping method: Karyolite BoBs

Other Genotyping (Cell Line)