SFC856-03-04 STING-/-F11D1
STBCi063-A-2
General
Cell Line |
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| hPSCreg name | STBCi063-A-2 |
| Cite as: | STBCi063-A-2 |
| Alternative name(s) |
SFC856-03-04 STING-/-F11D1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 28th November 2024 |
| User feedback | |
Provider |
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| Generator | University of Oxford (UOXF) |
| Owner | University of Oxford (UOXF) |
| Derivation country | United Kingdom |
External Databases |
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| BioSamples | SAMEA117399111 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104493790 |
Ethics
Also have a look at the ethics information for the parental line
STBCi063-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | ThermoFisher Cytotune |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
STBCi063-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Notes on reprogramming vector detection | See parent iPSC SFC856-03-04 STBCi063-A for vector clearance |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line STBCi063-A .Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal karyotype as per parent iPSC SFC856-03-04 STBCi063-A
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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