General

Cell Line
hPSCreg name	STBCi324-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	STBCi324-A-1
Alternative name(s)	SFC826-04-06 STING-/-B12
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	26th November 2024
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Provider
Generator	University of Oxford (UOXF)
Owner	University of Oxford (UOXF)
Distributors	University of Oxford (UOXF)
Derivation country	United Kingdom
External Databases
BioSamples	SAMEA117395648
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed Additional restrictions: MTA required from Oxford re edited subclone and from Luebeck re STBCi324-A parental line
Subclone of	STBCi324-A

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Parkinson Disease The donor is a carrier of a disease-associated mutation and affected. Synonyms Parkinson Disease Parkinson's disease Parkinson's Disease Genetic variants Nucleotide sequence variant in HGVS format NM_032409.3:c.1366C Is the variant homozygous or heterozygous? Homozygous
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA117389384

Ethics

Also have a look at the ethics information for the parental line STBCi324-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line STBCi324-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Is reprogramming vector detectable?	No
Methods used	PCR
Notes on reprogramming vector detection	see STBCi324-A
Vector free reprogramming
Other
Derived under xeno-free conditions	No
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line STBCi324-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes Normal Karyotype (C1 small LOH is same as parental cells) SFC826-04-06_STING_KO_B12.jpg Karyotyping method: Molecular karyotyping by SNP array http://
Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease

STING1 (target) Type of modification Gene knock-out Chromosome location 5q31.2 Free text Dual gRNA strategy to create deletion, confirmed by PCR across deletion. Differentiation to iPS-macrophages (along with WT which express STING) and Western Blot to confirm KO at protein level. Delivery method CRISPR-associated (CRISPR/Cas) System