SFC826-04-06 STING-/-B12
STBCi324-A-1
General
Cell Line |
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| hPSCreg name | STBCi324-A-1 |
| Cite as: | STBCi324-A-1 |
| Alternative name(s) |
SFC826-04-06 STING-/-B12
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
EDi001-A-2 (AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-3 (AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-4 (AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease CBIGi007-A-1 (IPSC0009, TMEM175 Q65Q homozygous correction) Donor's gene variants: TMEM175, TMEM175 Donor diseases: Parkinson Disease CBIGi007-A-2 (IPSC0010, TMEM175 P65P homozygous disease) Donor's gene variants: TMEM175, TMEM175 Donor diseases: Parkinson Disease CBIGi009-A-1 (IPSC0013, TMEM175 M393T homozygous correction) Donor's gene variants: TMEM175, TMEM175 Donor diseases: Parkinson Disease CBIGi013-A-1 (LRRK2 M1646T correction, IPSC0018) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi027-A-1 (IPSC0039, LRRK2 G2385R (CBIGi027) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease STBCi004-B-1 (SFC832-03-06 LRRK2WT/WT C47) Donor's gene variants: LRRK2 Donor diseases: Parkinson disease STBCi063-A-2 (SFC856-03-04 STING-/-F11D1) CBIGi039-A-1 (IPSC0051, LRRK2 R1441H(CBIGi039-A) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi041-A-1 (IPSC0054, GBA L444P (CBIGi041-A) correction) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease CBIGi042-A-1 (GBA E326K (CBIGi042-A) correction, IPSC0056) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease CBIGi044-A-1 (IPSC0059, LRRK2 N551K (CBIGi044-A) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi044-A-2 (IPSC0060, LRRK2 R1398H (CBIGi044-A) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease EDi001-A (AST22, AST23, SAMEA3319992) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: Parkinson disease |
| Last update | 26th November 2024 |
| User feedback | |
Provider |
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| Generator | University of Oxford (UOXF) |
| Owner | University of Oxford (UOXF) |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
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| BioSamples | SAMEA117395648 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
MTA required from Oxford re edited subclone and from Luebeck re STBCi324-A parental line |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA117389384 |
Ethics
Also have a look at the ethics information for the parental line
STBCi324-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
STBCi324-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Notes on reprogramming vector detection | see STBCi324-A |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line STBCi324-A .Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal Karyotype (C1 small LOH is same as parental cells)
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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