STJUDEi004-A-1

INS3 Isogenic

General

Cell Line

hPSCreg Name
STJUDEi004-A-1
Alternative name(s)
INS3 Isogenic
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th October 2022
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Provider

Generator St. Jude Children's Research Hospital (STJUDE)

External Databases

BioSamples SAMEA111437015
CLO CLO_0104089

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Diamond-Blackfan anemia (primary disease)
RPS19 c. 191T>C [p.Leu64Pro]
The donor is a carrier of a disease-associated mutation and affected.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA111437014

Ethics

Also have a look at the ethics information for the parental line STJUDEi004-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line STJUDEi004-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line STJUDEi004-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX, normal human female
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Diamond-Blackfan anemia
Genetic modifications
Isogenic modification
19q13.2
Heterozygous
RPS19 c.191T>C [p.Leu64Pro] mutation in the parental line has been fixed by Cas9/HDR-mediated repair, and correction verified by next-generation sequencing.
Repaired