INS14

General

Cell Line

hPSCreg name STJUDEi005-A
Cite as:
STJUDEi005-A (RRID:CVCL_C6SV)
Alternative name(s)
INS14
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 8th September 2023
Notes RPS19 c.184C>T [p.Arg62Trp]
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Provider

Generator St. Jude Children's Research Hospital (STJUDE)

External Databases

BioSamples SAMEA111437016
Cellosaurus CVCL_C6SV
Wikidata Q117704900

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
RPS19 mutation associated
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Aase syndrome
  • Aase-Smith II syndrome
  • Blackfan - Diamond syndrome
  • Blackfan-Diamond anaemia
  • Blackfan-Diamond anemia
  • DBA
  • Diamond-Blackfan anemia
  • chronic constitutional pure red cell anaemia
  • chronic constitutional pure red cell anemia
  • congenital PRCA
  • congenital hypoplastic anaemia
  • congenital hypoplastic anemia
  • congenital hypoplastic anemia, Blackfan-Diamond type
  • congenital pure red cell aplasia
  • erythrogenesis imperfecta
  • inherited erythroblastopenia
  • BDS
  • Blackfan Diamond syndrome
  • Red cell aplasia, pure hereditary
  • anaemia Diamond Blackfan type
  • anaemia congenital erythroid hypoplastic
  • anemia Diamond Blackfan type
  • anemia congenital erythroid hypoplastic
  • aregenerative anaemia chronic congenital
  • aregenerative anemia chronic congenital
show more synonyms
Disease associated phenotypes
  • Anemia

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XX, normal human female
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA111437017

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Marcin.wlodarski@stjude.org
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink marcin.wlodarski@stjude.org
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? St. Jude Children's Research Hospital Institutional Review Board #00000029
Approval number Pro00006262
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX, normal human female
Karyotyping method: G-Banding

Other Genotyping (Cell Line)