128Lp1

General#

Cell Line

hPSCreg Name SUSMi001-A-1
Alternative name(s)
128Lp1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Stanford University School of Medicine (SUSM)

External Databases

BioSamples SAMEA4909543
Cellosaurus CVCL_UM30

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Pelizaeus-Merzbacher disease
Synonyms
  • PMD
  • Pelizaeus-Merzbacher brain sclerosis
  • Sudanophilic leukodystrophy, Paelizeus-Merzbacher type
  • Diffuse familial brain sclerosis
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4909540

Ethics#

Also have a look at the ethics information for the parental line SUSMi001-A .
Alternatives to consent
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SUSMi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Notes on reprogramming vector detection Southern blot

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Pelizaeus-Merzbacher disease
Synonyms
  • PMD
  • Pelizaeus-Merzbacher brain sclerosis
  • Sudanophilic leukodystrophy, Paelizeus-Merzbacher type
  • Diffuse familial brain sclerosis
show more synonyms
Genetic modifications
Isogenic modification
Patient mutation in PLP1 gene was corrected to wildtype sequence.