128Lp1

General#

Cell Line

hPSCreg Name SUSMi001-A-1
Alternative name(s)
128Lp1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 21st June 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Stanford University School of Medicine (SUSM)

External Databases

BioSamples SAMEA4909543

General Information

Projects
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Pelizaeus-Merzbacher disease
Synonyms
  • PMD
  • Pelizaeus-Merzbacher brain sclerosis
  • Sudanophilic leukodystrophy, Paelizeus-Merzbacher type
  • Diffuse familial brain sclerosis
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4909540

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SUSMi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Notes on reprogramming vector detection Southern blot

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Pelizaeus-Merzbacher disease
Synonyms
  • PMD
  • Pelizaeus-Merzbacher brain sclerosis
  • Sudanophilic leukodystrophy, Paelizeus-Merzbacher type
  • Diffuse familial brain sclerosis
show more synonyms
Genetic modifications
Isogenic modification
Patient mutation in PLP1 gene was corrected to wildtype sequence.