RY3

General#

Cell Line

hPSCreg Name SUSMi001-A
Alternative name(s)
RY3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 21st June 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Stanford University School of Medicine (SUSM)

External Databases

BioSamples SAMEA4909539

General Information

Projects
Is the cell line available in principle? Available, without restrictions
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Pelizaeus-Merzbacher disease
Synonyms
  • PMD
  • Pelizaeus-Merzbacher brain sclerosis
  • Sudanophilic leukodystrophy, Paelizeus-Merzbacher type
  • Diffuse familial brain sclerosis
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4909540

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
skin of back
A zone of skin that is part of a back [Automatically generated definition].
Synonyms
  • back zone of skin
  • zone of skin of back
  • back skin
  • skin, dorsal region
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Notes on reprogramming vector detection Southern Blot

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal

Other Genotyping (Cell Line)