PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5

General#

Cell Line

hPSCreg Name LUBi001-A
Alternative name(s)
PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator H. Lundbeck A/S (LUB)
Distributors

External Databases

BioSamples SAMEA4096428
ECACC 66540226
Cellosaurus CVCL_VE41

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Frontotemporal dementia
The donor is affected.
Synonyms
  • FTD

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4096476

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 65-69

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Unkown
SSEA-1
Unkown
SSEA-4
Unkown
POU5F1 (OCT-4)
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Unkown
GSC
Unkown
FOXA2
Unkown
GATA6
Unkown
SOX17
Unkown
PITX1
Unkown
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
DCN
Unkown
NCAM1
Unkown
MIXL1
Unkown
VIM
Unkown
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
NeuroD1
Unkown
PAX6
Unkown
HES5
Unkown

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)