PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5

General

Cell Line

hPSCreg name LUBi001-A
Cite as:
LUBi001-A (RRID:CVCL_VE41)
Alternative name(s)
PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
LUBi001-B
(PGRN-8310, RCFB58 c4.4, PGRN8310, RCi194)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
LUBi001-C
(PGRN-8310, RCFB58 c3.7, RCi200, PGRN8310)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
UCLi004-A
(RCi173, RCFB60c6)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-B
(RCFB60c7, RCi177)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-C
(RCi172, RCFB60c2)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi009-A
(CB1D)
Donor's gene variants:
VCP
Donor diseases:
Frontotemporal dementia
UCLi010-A
Donor's gene variants:
VCP
Donor diseases:
Frontotemporal dementia
UCLi010-B
(GL1B)
Donor's gene variants:
VCP
Donor diseases:
Frontotemporal dementia
UCLi001-A
(HHItC9S-V19)
Donor's gene variants:
C9orf72
Donor diseases:
Frontotemporal dementia
UCLi002-A
(HHItC9D-V34, DN19)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Frontotemporal dementia
UCLi009-B
(CB1E)
Donor's gene variants:
VCP
Donor diseases:
Frontotemporal dementia
UCLi003-A
(TSM(exon10+16)V97)
Donor's gene variants:
MAPT
Donor diseases:
Frontotemporal dementia
STBCi034-A
(SFC091-04-03)
Donor diseases:
migraine disorder
STBCi046-A
(SFC021-03-01)
Donor diseases:
migraine disorder
STBCi034-B
(SFC091-04-01)
Donor diseases:
migraine disorder
STBCi046-B
(SFC021-03-02)
Donor diseases:
migraine disorder
STBCi071-C
(SFC057-07-02)
Donor diseases:
Alzheimer disease
STBCi090-B
(SFC867-04-12)
Donor diseases:
Parkinson disease
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi028-A
(#28c3, CHDI-90002176)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-B
(SFC801-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-C
(SFC801-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
CHDIi035-A
(#108c5, CHDI-90002183)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
CHDIi036-A
(#110c5, CHDI-90002184)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi254-A
(SFC143-03-08)
Donor diseases:
Alzheimer disease
CHDIi038-A
(#112c7, CHDI-90002186)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
WTSIi614-A
(HPSI0616i-rily_3)
Donor diseases:
Spastic paraplegia
CHDIi040-A
(#115c6, CHDI-90002188)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi258-A
(SFC090-03-07)
Donor diseases:
Parkinson disease
STBCi259-A
(SFC032-03-07)
Donor diseases:
Parkinson disease
CHDIi045-A
(#121c6, CHDI-90002193)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi046-A
(#122c1, CHDI-90002194)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi015-A
(SFC809-03-01)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi265-A
(SFC121-03-02)
Donor diseases:
Parkinson disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi267-A
(SFC027-03-02)
Donor diseases:
Parkinson disease
STBCi268-A
(SFC077-03-04)
Donor diseases:
Parkinson disease
WTSIi472-B
(HPSI0216i-vieg_3)
Donor diseases:
Hereditary ataxia
WTSIi448-B
(HPSI0316i-muwv_6)
Donor diseases:
Hereditary ataxia
Last update 22nd May 2019
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Provider

Generator H. Lundbeck A/S (LUB)
Distributors

External Databases

BioSamples SAMEA4096428
Cellosaurus CVCL_VE41
Wikidata Q54903225

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 65-69

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • FTD
Genetic variants
GRN (target)
17q21.31
NM_002087.3:c.1477C>T
NP_002078.1:p.Arg493Ter
Progranulin: R493X mutation

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4096476

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Selina Wray
Alternatives to consent
Is there other documentation provided to the donor for consenting purposes? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? No
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Institute of Neurology Joint Research Ethics Committee
Approval number 09/H0716/64
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Institute of Neurology Joint Research Ethics Committee
Approval number 09/H0716/64
Do you have obligations to third parties in regard to the use of the cell line? No
Please describe:
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Further constraints on use

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 65-69

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Unknown
GSC
Unknown
FOXA2
Unknown
GATA6
Unknown
SOX17
Unknown
PITX1
Unknown
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
DCN
Unknown
NCAM1
Unknown
MIXL1
Unknown
VIM
Unknown
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
NeuroD1
Unknown
PAX6
Unknown
HES5
Unknown

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: P22
Karyotyping method: G-Banding

Other Genotyping (Cell Line)