HHItC9D-V34, DN19

General#

Cell Line

hPSCreg Name UCLi002-A
Alternative name(s)
HHItC9D-V34, DN19
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 4th October 2017

Provider

Generator University College London (UCL)
Distributors EBiSC (UCLi002-A on EBiSC)

External Databases

BioSamples SAMEA3174461
Cellosaurus CVCL_9S55

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Frontotemporal dementia
The donor is affected.
Synonyms
  • FTD
Genetic variants
C9ORF72 (target)
9p21.2
There is a mutation present however this is not a SNP but is an expansion therefore the data cannot be entered. The identified mutation is Hexanucleotide repeat expansion, the gene is C9ORF72 and the location is 9p21.2.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA3174486

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hIPSC Derivation#

General

Primary cell type Fibroblast of dermis
Fibroblast of dermis in the OLS
Dermal Fibroblast
Primary cell line name
Primary cell line vendor
Age of donor at collection 55-59

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
GSC
No
GATA6
Yes
CXCR4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
GATA4
Yes
PITX1
Yes
DECORIN
Yes
PECAM1
Yes
PDGFRA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
BUTUBULIN
Yes
NEUROD1
Yes
HES5
Yes
FOXG1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)