UGOTSAi006-A

N1-005iC1

General

Cell Line

hPSCreg name UGOTSAi006-A
Cite as:
UGOTSAi006-A (RRID:CVCL_C1XB)
Alternative name(s)
N1-005iC1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UMi042-A-1
(408737 CB9)
Donor diseases:
Alzheimer's Disease
UGOTSAi002-B
(N1-001iC2)
Donor diseases:
Alzheimer's Disease
UGOTSAi003-A
(N1-002iC3A)
Donor diseases:
Alzheimer's Disease
UGOTSAi003-B
(N1-002iC4)
Donor diseases:
Alzheimer's Disease
UGOTSAi004-A
(N1-003iC3)
Donor diseases:
Alzheimer's Disease
UGOTSAi004-B
(N1-003iC4)
Donor diseases:
Alzheimer's Disease
STBCi006-A
(SFC140-04-01)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi097-C
(SFC055-04-03)
Donor diseases:
Alzheimer disease
STBCi099-A
(SFC059-03-01)
Donor diseases:
Alzheimer disease
STBCi099-B
(SFC059-03-02)
Donor diseases:
Alzheimer disease
STBCi134-A
(SFC019-03-01)
Donor diseases:
Alzheimer disease
MUNIi013-A
(BAD4, sAD3)
Donor diseases:
Alzheimer's Disease
IBTCMi004-A
(AD2)
Donor diseases:
Alzheimer's Disease
IBTCMi005-A
(AD3)
Donor diseases:
Alzheimer's Disease
STBCi071-C
(SFC057-07-02)
Donor diseases:
Alzheimer disease
ZZUi013-A
(ZZU-iPS-AD-MEOX2-001)
Donor diseases:
obsolete_Alzheimer's disease
STBCi097-A
(SFC055-04-02)
Donor diseases:
Alzheimer disease
STBCi097-B
(SFC055-04-01)
Donor diseases:
Alzheimer disease
Last update 19th December 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator The Sahlgrenska Academy at University of Gothenburg (UGOTSA)
Owner The Sahlgrenska Academy at University of Gothenburg (UGOTSA)
Derivation country Sweden

External Databases

BioSamples SAMEA110104322
Cellosaurus CVCL_C1XB
Wikidata Q114313161

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Neurodegeneration, Alzheimer's disease
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

Cells and samples deriving from cells must be traceable at all times. Cells and samples derived from cells must be destroyed upon donor's request.

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 70-74

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Alzheimer's Disease
  • Alzheimer disease
  • Alzheimer Disease
  • Alzheimer dementia
  • Alzheimer's Dementia
  • Alzheimer's disease
  • Alzheimer's disease, unspecified
show more synonyms
Is the medical history available upon request? No
Is clinical information available? N0

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA110104323

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project To study disease mechanisms related to neurodegeneration and to test drug candidates in the relevant field.
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? No
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Regionala etikprövningsnämnden i Göteborg (EPN)
Approval number 731-14
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Regionala etikprövningsnämnden i Göteborg (EPN)
Approval number 731-14
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Stemcell techologies
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
Source cell origin
Either of the fleshy mounds in the rear pelvic area of the human body formed by the gluteal muscles.
Synonyms
  • BUTTOCK
  • Buttock
  • buttock
  • clunis
  • gluteal part of pelvic girdle
  • gluteal region
  • regio glutealis
show more synonyms
Age of donor (at collection) 70-74
Collected in 2020
Passage number reprogrammed 2

Reprogramming method

Vector type Non-integrating
Vector ReproRNA™-OKSGM
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Selection criteria for clones Clones consisting of iPSCs only, large enough to be picked manually and with a sharp edge towards other cells were selected.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
N-cadherin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal karyotype; 46, XY
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)